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A complex of early Gothic buildings and churches dating from the 13th century, the convent, tucked in a quiet corner of Staré Mesto, began exhibiting much of the National Gallery's collection of medieval art in 2000. Once home to the Order of the Poor Clares, it was established in 1234 by St. Agnes of Bohemia, sister of Wenceslas I. The Blessed Agnes became St. Agnes when Pope John Paul II paid his first visit to Prague in 1990 for her canonization.

The most famous among the unique collection of Czech Gothic panel paintings are those by the Master of the Hohenfurth Altarpiece and the Master Theodoricus. The convent is at the end of Anezka, off Hastalské námestí.